Transcriptome analysis of Wild Type and Mir-212/132ILN/ILN mouse mammary gland

Mice deficient for miR-212/132 have been reported to show impaired mammary gland development. However, another miR-212/132-deficient line does not demonstrate any obvious defects in mammary gland organogenesis. The transcriptome analysis in the mammary gland of the previously reported miR-212/132-deficient line by deep RNA-seq revealed significantly deregulated expression of genes flanking Mir-212/132 locus, such as HIC1, implying that the mammary gland phenotype might not be only due to loss of miR-212/132 Mammary gland mRNA profiles of Wild Type and Mir-212/132ILN/ILN mice were generated by deep sequencing using Illumina GAIIx.已有研究显示，缺失微小RNA-212/132（miR-212/132）的小鼠会出现乳腺发育受损的表型。然而，另一株miR-212/132缺陷小鼠品系在乳腺器官发生过程中未表现出任何明显异常。通过深度RNA测序（deep RNA-seq）对前述报道的miR-212/132缺陷小鼠品系的乳腺组织开展转录组分析，结果显示Mir-212/132基因座侧翼的基因（如HIC1）表达存在显著失调，提示该小鼠的乳腺表型可能并非仅由miR-212/132的缺失所导致。本数据集采用Illumina GAIIx测序平台（Illumina GAIIx）对野生型（Wild Type）及Mir-212/132ILN/ILN小鼠的乳腺mRNA进行了深度测序，进而获取其转录组表达谱。