Aberrant DNA Methylation and Overexpression of NR0B1 are Prognostic Biomarkers in KEAP1-Mutant Lung Adenocarcinomas

This dataset contains bisulfite Sanger sequencing results for a specific CpG-rich fragment of the NR0B1 gene promoter, analyzed in two human cell lines: A549 (lung adenocarcinoma) Calu-3 (lung epithelial) The purpose of this analysis was to evaluate the methylation status of individual CpG sites within this region. Included Files: .ab1 files: raw chromatogram outputs from direct bisulfite sequencing Excel spreadsheet summarizing methylation levels at each CpG site for both samples Reference sequence and protocol documentation Methylation Level Codes: 0 = Unmethylated 1 = <33% methylation 2 = 33–67% methylation 3 = >67% methylation 4 = ~100% methylation ? = Indeterminable Sequencing was performed using standard bisulfite PCR and BigDye 3.1 chemistry, with capillary electrophoresis carried out on an ABI 3730XL instrument. Data were analyzed using Polyphred and manually reviewed.