CTD profiles collected with RV Connecticut on the Continental shelf and slope south of Montauk, NY on 14-15 June 2022.

Seven stations on the shelf and slope south of Montauk, NY on 14-15 June 2022 were sampled. Basic hydrographic properties, including temperature, salinity, dissolved oxygen, and pH were measured and rosette samples were taken for chlorophyll, nutrients, and microplankton. Water was filtered for metagenomics and metatranscriptomics and picked individual microplankters for sequencing. Three to ten depths were sampled at each station. Our shallowest station was on the shelf at 38m and the deepest was at the slope edge at 2400m. Due to limitations of the CTD our deepest sampled depth was 1150m. At the two shallowest stations, we also deployed a Wetstar fluorometer for phytoplankton fluorescence. The CTD data were processed with SeaBird's Seasoft program and converted to spreadsheet format.2022年6月14日至15日，研究人员对美国纽约州蒙托克（Montauk）以南陆架与大陆斜坡区域的7个站位完成采样作业。本次测定了水温、盐度、溶解氧及pH值等基础水文参数，同时利用罗塞特采水器（rosette sampler）采集了用于叶绿素、营养盐与微型浮游生物（microplankton）分析的水样；对部分水体进行过滤以开展宏基因组学（metagenomics）与宏转录组学（metatranscriptomics）测序，并挑取单个微型浮游生物个体进行测序。每个站位设置3至10个采样深度，本次采样的最浅站位位于陆架区，水深38米，初始计划的最深站位设于大陆斜坡边缘，水深2400米，但受温盐深仪（CTD，Conductivity-Temperature-Depth）性能限制，实际最大采样水深为1150米。在两个最浅站位，研究人员还部署了Wetstar荧光计（Wetstar fluorometer）以检测浮游植物荧光信号。CTD数据通过海鸟科技（SeaBird）的Seasoft软件完成处理，并转换为电子表格格式。