Single-cell epigenomic analysis of hypodermal macrophages from Csf1r-cre x Igf1-flox mice.
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https://www.ncbi.nlm.nih.gov/sra/SRP428504
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To explore the effect of cell-autonomous Igf1 expression on hypodermal macrophages, these cells were isolated from Csf1r-cre x Igf1-flox mice and subjected to single-cell ATAC-sequencing. Overall design: Skins from Csf1r-cre x Igf1-flox and Igf1-flox mice were harvested and the hypodermal layers isolated by mechanical force and enzymatic treatments. Cells were liberated after enzymatic digestion of the tissues. Macrophages were isolated by fluorescence-activated cell sorting (FACS) and their chromatin accessibility assessed by single-cell ATAC-sequencing using the 10X genomics plateform.
创建时间：
2023-09-07
