Proteins co-enriched with Piccolo.

Two protocols to biochemically enrich for Piccolo and other CAZ proteins (column chromatography & immuno-precipitation) were subjected to MudPIT to identify co-purifying proteins. The most interesting proteins would fulfill two criteria: (1) They would be detected in the 290 mM NaCl fraction, but not in the 210 mM or 350 mM fractions; (2) They would be detected in the Piccolo antibody (Ab) co-IP, but not in the control IgG IP. Piccolo, Bassoon, and Trio were the only three proteins that fulfilled these criteria. Also shown in this table for comparison are the peptides identified for several CAZ proteins and Piccolo/Bassoon interacting proteins. For each protein in each biochemical fraction are shown the number of Distributed Spectral Counts (dSpC) identified, along with the Distributed Normalized Spectral Abundance Factor (dNSAF)[48].