Screening aptamer switches for ATP and glucose

Aptamer-based molecular switches that undergo a binding-induced conformational change have proven valuable for a wide range of applications, such as imaging metabolites in cells, targeted drug delivery, and real-time detection of biomolecules. Here, we describe a massively parallel screening-based strategy that enables the conversion of virtually any aptamer into a molecular switch. Using this approach, we generate aptamer switches towards ATP and glucose.