RNA-seq of cochlea in mice with a frameshift mutation in the reading frame of thyroid hormone receptor alpha1

Thyroid hormone is known to be required for inner ear development. The influence of the thyroid hormone receptor alpha1 is however unclear, as most previous studies point to a predominant function of the TRbeta1/2 isotypes. We report a RNA-seq analysis which completes a full histological and functional analysis showing that mice carrying a mutation of the TRalpha1 receptor have mild alteration of inner ear. Whole inner ear RNA was extracted from heterozygous mutant mice carrying a frameshift mutation which alters the function of TRalpha1. These RNA were compared to RNA extracted from wild-type littermates.已知甲状腺激素（Thyroid hormone）是内耳发育所必需的调控因子。然而，甲状腺激素受体α1（thyroid hormone receptor alpha1）的具体调控作用尚不明确，因既往多数研究均指出TRβ1/2亚型（TRbeta1/2 isotypes）发挥主要功能。本研究报道了一项RNA测序（RNA-seq）分析，该分析结合全面的组织学与功能学验证，证实携带TRα1受体（TRalpha1 receptor）突变的小鼠存在轻度内耳异常。研究人员从携带可破坏TRα1功能的移码突变的杂合突变小鼠体内提取了全内耳总RNA，并将其与同窝野生型小鼠的总RNA进行了对照分析。