TDP-43 regulates miRNA biogenesis and cytotoxicity by obstructing Dicer activity

The cytoplasmic Dicer proteins play a critical role in miRNA biogenesis. Deficiency of TDP-43 fly homologs, TBPH, and expression of its A315T pathogenic mutations reduces the levels of Drosophila dicer proteins (DCR-1 and DCR-2) in ALS fly models. Figure 1A and Figure 5A contain the original image files of Western Blot that were used to evaluate the expression levels of miRNA biogenesis-related proteins (including DCR-1 and DCR-2) in TBPH KO (compare to W- and heterozygous, Figure 1A) and TBPH A315T mutant (compare to TBPH WT and LacZ control, Figure 5A), flies. The files "W-vs-TBPHdelta23-mRNA.filter.annot.xls.zip" and "W-vs-TBPHdelta23-miRNA.filter.xls.zip" contain the miRNA and RNA reference sequencing data, including annotations and expression levels, from TBPH KO and W- control flies.细胞质Dicer蛋白（Dicer）在miRNA生物发生（miRNA biogenesis）过程中发挥关键作用。TDP-43的果蝇同源蛋白TBPH缺失，以及其A315T致病性突变体的表达，会降低肌萎缩侧索硬化（ALS）果蝇模型中果蝇Dicer蛋白（DCR-1与DCR-2）的表达水平。图1A与图5A包含了用于评估TBPH敲除（TBPH KO，与野生型对照W-及杂合子相比，图1A）以及TBPH A315T突变体果蝇（与TBPH野生型（TBPH WT）及β-半乳糖苷酶（LacZ）对照相比，图5A）体内miRNA生物发生相关蛋白（包括DCR-1与DCR-2）表达水平的蛋白质免疫印迹（Western Blot）实验原始图像文件。压缩文件"W-vs-TBPHdelta23-mRNA.filter.annot.xls.zip"与"W-vs-TBPHdelta23-miRNA.filter.xls.zip"包含了来自TBPH敲除果蝇与W-对照果蝇的miRNA及RNA参考测序数据，涵盖注释信息与表达水平。