scRNA-seq profiling of vascular cells isolated from disease-free human breast based on the expression of CD36 and/or CD31

To identify markers preferentially expressed by capillary ECs (highly expressing CD36) in the disease-free human breast and gain insights into their biology, we used single cell RNA sequencing (scRNA-seq). For this analysis, dissociated cells from disease-free tissues were barcoded using the MULTI-seq protocol and pooled prior to FACS-based isolation of CD36+ and/or CD31+ cells. The droplet-based 10x Genomics Chromium platform was used to encapsulate single cells and construct cDNA libraries. Populations of fibroblasts, perivascular, lymphatic and vascular endothelial ECs where identified in this analysis. CD36 and/or CD31 expressing cells (labled with unique lipid-modified oligonucleotides barcodes) were isolated from Human breast tissue (14 donors) by FACS and subjected to scRNAseq using the 10X Genomics Single Cell V2 platform. NOTE from submitter: the human tissues were collected under an approved protocol that has not been updated to consent the publication of raw sequencing data.