Expression characteristics of <i>CsPG23</i> in citrus and analysis of its interacting protein

Exploring the resistance genes of citrus to Huanglongbing (HLB) is the foundation and key to citrus disease-resistant breeding. Through the analysis of comparative transcriptome data, we identified six cell wall degradation genes that respond to citrus infection with <i>Ca</i>Las. We selected one of the genes with high differential expression levels and cloned it, naming it <i>CsPG23</i>. The subcellular localization results of tobacco indicated that the <i>CsPG23</i> protein is localized in the nucleus, cytoplasm, and cell membrane. Real-time fluorescence quantitative PCR (RT-qPCR) analysis showed that the expression of <i>CsPG23</i> is related to variety tolerance, tissue location, and symptom development. In addition, we constructed overexpression and silencing vectors for <i>CsPG23</i> and obtained <i>CsPG23</i> silencing plants, overexpression and silencing hairy roots, and analyzed the expression characteristics of <i>CsPG23</i> in response to SA, JA, MeSA and H₂O₂ induction through RT-qPCR. Using Protein–Protein Interaction (PPI) to predict and screen for a citrus protein CsAGD8 that may interact with CsPG23, and preliminarily verifying its interaction with CsPG23 protein through Yeast Two-hybrid (Y2H). We constructed overexpression and silencing vectors for <i>CsAGD8</i> and obtained <i>CsAGD8</i> overexpression and silencing hairy roots. In summary, it is indicated that CsPG23 may interact with CsAGD8 in response to <i>Ca</i>Las infection.