Adenylosuccinate lyase (ADSL) enhances endometrial cancer aggressiveness via increasing killer cell lectin like receptor C3 expression by fumarate

Although ADSL is an enzyme that is involved in de novo purine synthesis, it was reported that ADSL knockdown did not result in decreases in adenine nucleotides (AMP, ADP, and ATP) or GTP. Then, we hypothesized that decreased cell proliferation, migration and invasion capability by ADSL knockdown in endometrial cancer cells were caused through a mechanism independent of purine synthesis. To elucidate a mechanism, we performed DNA microarray-based gene expression profiling using ADSL knockdown and control HEC1B cells. In the analysis, 940 out of 34,127 probes showed greater than two-fold changes in expression with a significant difference, p value < 0.05. The labeled cRNAs were hybridized on a Whole Human Genome Oligo Microarray ver. 2 (G4845A) in two dye swap experiments, resulting in four individual microarrays.