Fluorescent Protein Analysis Methodology

This archive file contains the files required for the fluorescent protein spectral analysis. The input file Wounding_Aaspera_fl.csv containing the raw fluorescence values and sample protein concentration is provided along with the reference fluorescent protein data file modelspecs_interp_25sh.csv (based on the spectrum of purified Acropora millepora fluorescent proteins) and the spectral decomposition script specs_regression_WF.R. The reference fluorescent protein data and the script were provided by Mikhail Matz.本压缩归档文件包含荧光蛋白（fluorescent protein）光谱分析所需的全部相关文件。本次一并提供了包含原始荧光值与样品蛋白浓度数据的输入文件Wounding_Aaspera_fl.csv，同时附带参考荧光蛋白数据集文件modelspecs_interp_25sh.csv（基于纯化的微孔鹿角珊瑚（Acropora millepora）荧光蛋白的光谱），以及光谱分解脚本specs_regression_WF.R。上述参考荧光蛋白数据集及分析脚本均由米哈伊尔·马茨（Mikhail Matz）提供。