Effect of silencing Rae1 or Nup98 expression on RNA synthesis in VSV-infected cells.
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HeLa cells transfected with the indicated siRNA were either mock-infected or infected with VSV in the presence or absence of actinomycin D. At 6 h postinfection, cells were labeled with 3H uridine for 30 min; RNA was acid-precipitated, and radioactivity was determined by scintillation counting. Data shown are mean ± s.d. for triplicate cultures from a representative experiment.aAbbreviations: ActD - actinomycin D; NT – non-targeting siRNA.
创建时间：
2015-12-02
