Efficient production of polyhydroxyalkanoates (PHAs) from <i>Pseudomonas mendocina</i> PSU using a biodiesel liquid waste (BLW) as the sole carbon source

Conditions for the optimal production of polyhydroxyalkanoate (PHA) by <i>Pseudomonas mendocina</i> PSU using a biodiesel liquid waste (BLW) were determined by response surface methodology. These were an initial carbon to nitrogen ratio (C/N) of 40 (mole/mole), an initial pH of 7.0, and a temperature of 35 °C. A biomass and PHA concentration of 3.65 g/L and about 2.6 g/L (77% DCW), respectively, were achieved in a growth associated process using 20 g/L glycerol in the BLW after 36 h of exponential growth. The PHA monomer compositions were 3HB (3-hydroxybutyrate), a short-chain-length-PHA, and the medium-chain-length-PHA e.g. 3-hydroxyoctanoate and 3-hydroxydecanoate. Both the <i>phbC</i> and <i>phaC</i> genes were characterized. The <i>phbC</i> enzyme had not been previously detected in a <i>Pseudomonas mendocina</i> species. A 2.15 g/L of an exopolysaccharide, alginate, was also produced with a similar composition to that of other <i>Pseudomonas</i> species. PHA biosynthesis from biodiesel liquid waste (BLW) by <i>Pseudomonas mendocina</i> PSU.