Flow cytometry data – LA01: dual-parameter replicative age and viability profiling of Saccharomyces cerevisiae var. chevalieri
收藏资源简介:
LA01 — Saccharomyces cerevisiae var. chevalieri Strain: SafBrew™ LA-01 (Saccharomyces cerevisiae var. chevalieri), industrial yeast for low-alcohol beer production. Role: application dataset. Data: 30 FCS files. Measurements performed in ICP buffer with three biological replicates per condition. Three measurement conditions: mCherry fluorescence without dye (Age_I, 12 files, pH 3–6), mCherry fluorescence with CFDA staining (Age_I_CFDA, 12 files, pH 3–6), and unstained reference controls (R, 6 files, pH 3 and 4 only). Analysis: Identical workflow to BE134, scale group B (FITC-A/PerCP-A threshold 10.0). Age_I files at pH 6 are included in the dataset but were excluded from analysis to maintain parallel structure with BE134. No MOPS buffer measurements were performed.



