ATAT_seq of Trm from B16 tumours of Scml4fl/fl and Cd8-Cre;Scml4fl/fl mice

chromatin sequencing (ATAC-seq) on Trm cells from Scml4fl/fl and Cd8-Cre;Scml4fl/fl mice bearing B16 melanoma tumours to map open chromatin regions and identify ATAC peaks located in regions between promoters, introns, and distal genes. Trm were isolated from B16 melanoma tumours of mice. Unfixed nuclei in these cells were labelled with tn5 transposase (TruePrep DNA Library Prep Kit V2 for Illumina) by incubation for 30 min at 37 C. Then, library fragments were generated by 10-12 cycles of PCR amplification and finally sequenced on the Illumina HiSeq X Ten platform (PE150). Reads were mapped to the NCBI37 (mm9) murine reference genome and annotated to the nearest gene TSS using esATAC.