RNA-sequencing analysis gene expression changes in response to ischemia reperfusion and upon manipulation of miR-483-5p in PC12 highly differentiated cells

The consequences of cardiac arrest are often fatal, including brain injury after resuscitation. It has been reported that few people patients can recover to the neurological state before cardiac arrest. MiRNAs are short non-protein-coding RNA molecules that are evolutionarily conserved and ubiquitously expressed. Numerous pieces of research have reviewed the role of miRNAs in regulating neuronal apoptosis, regeneration, and plasticity of neurons, and inflammatory after cardiac arrest . As the stability of miRNAs in the bloodstream and the function in the regulation of neurological impairment after ischemia-reperfusion injury, microRNAs have been the most potential new biomarkers and therapeutic targets after cardiac arrest to alleviate neurological impairment . In this work, we found that the level of miR-483-5p is correlated to the prognosis of neurological function. To investigate the function of miR-483-5p on neurons after ischemia-reperfusion injury,we established highly differentiated PC12 cell lines in which miR-483-5p was overexpressed by transfection with miR-483-5p mimcis.We then performed gene expression profiling analysis using data obtained from RNA-seq of PC12 cells in different groups. Highly differentiated PC12 cells were used as the research objects.Comparative gene expression profiling analysis of RNA-seq data for PC12 cells ,PC12 cells after oxygen glucose deprivation/reperfusion treatment and PC12 cells transfected with miR-483-5p mimcis after OGD/R treatment .There are three duplicate samples in each group. The divided experimental groups was as follows: control rep1 ,control rep2,control rep3,OGD/R rep1,OGD/R rep2, OGD/R rep3, OGD/R +miR-483-5p mimcis rep1, OGD/R +miR-483-5p mimcis rep2,OGD/R +miR-483-5p mimcis rep3.