miR-30 Data for Figures

Data File 1. Plasmid Sequences
Figure 1 data. Cells were transfected with an miR-30ac2 expression plasmid (“G30ac2”) or a control plasmid (“Gscr”). RNA was isolated and qRT-PCR was performed to measure the indicated transcripts. Expression was quantified using the delta-delta method.
Figure 2 data. Cells were transfected with the miR-30ac2 expression plasmid “pSLIKG-miR30ac2”) or a control plasmid (“pSLIKG-Scr”), plus the indicated luciferase reported plasmid. Renilla and firefly luciferase activities were measured in cell exctracts. The firefly luciferase activity was used to normalize for transfection efficiency, and further correction was performed to control for the effects of the reporter plasmids and the miRNA expression plasmid. Comparison was made for each reporter plasmid between the miR-30ac2 expression plasmid and the control plasmid, and averages, standard error, and p-values were calculated.
Figure 3 data. Table of PITA and Targetscan scores (if available) for the indicated UTRs, with the corresponding luciferase expression data from Figure 2.

数据文件1：质粒序列
图1数据：细胞被转染以表达miR-30ac2的质粒（命名为“G30ac2”）或对照组质粒（命名为“Gscr”）。通过RNA提取和实时定量PCR技术检测所指示的转录本表达水平。采用delta-delta法对表达量进行量化。
图2数据：细胞被转染以表达miR-30ac2的质粒“pSLIKG-miR30ac2”或对照组质粒“pSLIKG-Scr”，以及指定的荧光素报告质粒。在细胞提取物中测量了Renilla和萤火虫荧光素酶的活性。萤火虫荧光素酶活性被用作转染效率的标准化指标，并进一步校正以控制报告质粒和miRNA表达质粒的影响。对每个报告质粒，在miR-30ac2表达质粒与对照质粒之间进行对比，并计算平均值、标准误和p值。
图3数据：对于所指示的UTRs，列出了PITA和Targetscan评分（如有），以及图2中对应的荧光素酶表达数据。