LncRNA expression data from human plasma of control donors and chronic HF patients

There are a lot of non-coding RNAs in human plasma. Some of them could be a biomarker of diseases. We used microarrys to detect lncRNAs in plasma of 10 control subjects and 10 heart failure (HF) patients diagnosed with dilated cardiomyopathy (DCM) and we identified distinct classes of dysregulated lncRNAs. Blood samples were collected from 10 control donors (victims of accident) and 10 chronic HF patients diagnosed with DCM during heart transplantation surgery. Then, plasma was obtained by centrifugation at 2000g at 4ºC for 10 minutes, and the supernatant was carefully transferred to a new RNAse/DNAse-free 1.5mL microtube and stored at -80ºC until use. Total RNA was extracted from 250ul plasma by TRIzol LS Reagent (Life Technologies, Carlsbad, CA) following the instructions. RNA was pre-amplified and then underwent microarray analysis (Arraystar, Human lncRNA array, version 3.0).