datatodryad

This Excel file contains the main data from Fuller & Claricoates. We list the population (population), the water treatment (water), and the day on which fish were sampled after being introduced into the lighting treatments (day). Due to low sample sizes, we had to pool these into “early, middle, and late” sampling periods which we refer to as (time). We also list the (sample name) and (sex) of the individual. We then list the mean critical threshold cycle for each individual for the following genes: elongation factor 1-alpha (CT-ef 1a), LWS (CT-LWS), RH2-1 (CT-RH2-1), RH2-2 (CT-RH2-2), SWS1 (CT-SWS1), SWS2A (CT-SWS2A), and SWS2B (CT-SWS2B). Based on these data and the efficiencies listed in our manuscript, we calculated the expression of each opsin gene relative to our housekeeping gene, elongation factor 1 alpha. These are listed as (rel-lws_ef), (rel-rh2-1_ef), (rel-rh2-2_ef), (rel-sws1_ef), (rel-sws2a_ef), and (rel-sws2b_ef). We also calculated the natural log of the relative expression of each opsin. These are listed in this file as (log(relsws1-ef)), (log(relsws2b-ef)), (log(relsws2a-ef)), (log(relrh21-ef)), (log(relrh22-ef)), and (log(rellws-ef)). We also calculated the proportional expression of each opsin gene relative to the total opsin pool. These are listed in this file as (propLWS), (propRH2-1), (propRH2-2), (propSWS1), (propSWS2a), and (propSWS2b). Finally, we also calculated the proportion expression of each opsin gene relative to all of the opsins, but excluding RH2-2. These are listed in this file as (propSWS1(noRH2-2)), (propSWS2b(noRH2-2)), (propSWS2a(noRH2-2)), (propRH2-1(noRH2-2)), and (propLWS(noRH2-2)). For other inquiries about these data, please contact Becky Fuller – [e-mail].本Excel文件收录了Fuller与Claricoates研究的核心数据集。本文件记录了种群（population）、水处理条件（water treatment），以及鱼被引入光照处理后至采样的天数（day）。由于样本量偏低，我们将采样样本合并为“早期、中期、晚期”三个采样阶段，并以（time）指代该分类。此外，我们还记录了每条个体的样本名称（sample name）与性别（sex）。 随后，我们列出了每条个体针对下述基因的平均临界阈值循环数（CT值）：延伸因子1-α（elongation factor 1-alpha，对应CT值为CT-ef 1a）、LWS（对应CT值为CT-LWS）、RH2-1（对应CT值为CT-RH2-1）、RH2-2（对应CT值为CT-RH2-2）、SWS1（对应CT值为CT-SWS1）、SWS2A（对应CT值为CT-SWS2A）以及SWS2B（对应CT值为CT-SWS2B）。 基于上述数据与论文中公布的扩增效率，我们计算了各视蛋白（opsin）基因相对于持家基因（housekeeping gene）延伸因子1-α的相对表达量，对应字段分别为(rel-lws_ef)、(rel-rh2-1_ef)、(rel-rh2-2_ef)、(rel-sws1_ef)、(rel-sws2a_ef)及(rel-sws2b_ef)。 我们还计算了各视蛋白相对表达量的自然对数，对应字段依次为(log(relsws1-ef))、(log(relsws2b-ef))、(log(relsws2a-ef))、(log(relrh21-ef))、(log(relrh22-ef))及(log(rellws-ef))。 此外，我们还计算了各视蛋白基因相对于视蛋白总库的比例表达量，对应字段为(propLWS)、(propRH2-1)、(propRH2-2)、(propSWS1)、(propSWS2a)及(propSWS2b)。 最后，我们还计算了仅以排除RH2-2后的所有视蛋白为参照时，各视蛋白基因的比例表达量，对应字段为(propSWS1(noRH2-2))、(propSWS2b(noRH2-2))、(propSWS2a(noRH2-2))、(propRH2-1(noRH2-2))及(propLWS(noRH2-2))。 若需查询该数据集的其他相关信息，请联系Becky Fuller，邮箱地址为[e-mail]。