Anion-Exchange Chromatography of Phosphopeptides: Weak Anion Exchange versus Strong Anion Exchange and Anion-Exchange Chromatography versus Electrostatic Repulsion-Hydrophilic Interaction Chromatography.

Data from ProteomeXchange, PXD ID: PXD001333. File: Amanda ms1-8ppm ms2-20ppm 20130219_QEx3_RSLC-4_Mischerikow_Peters_IMP_shotgun_s2324.msf.mgf. Published as part of Anal Chem. 2015 Apr 17 . From the Abstract: {{i}} ... Weak anion-exchange (WAX) and strong anion-exchange (SAX) columns were compared, with both peptide standards and a HeLa cell tryptic digest. The SAX column exhibited greater retention at pH 6 than did the WAX column. However, only about 60% as many phosphopeptides were identified with SAX at pH 6 than via ERLIC at pH 2 ... {{/i}}