Establishing Guideline Values for Copper Toxicity to Marine Microbiomes

This data collection consists of water quality measurements, nutrient data and measured copper concentrations associated with an experiment exploring the effects of copper on seawater microbial communities to establish guideline values for copper toxicity to seawater microbiomes. Seawater for the experiment was collected from the AIMS jetty (19°16'38.4"S 147°03'32.1"E) in December 2020 and subsequently used in a 48-h exposure experiment. Water quality measurements (dissolved oxygen, pH and salinity) and water samples for nutrients (dissolved organic / inorganic carbon and nitrogen) were taken at 0-h and 48-h, following standard AIMS procedures. Representative water samples from the nominal experimental treatments (0, 0.1, 0.3, 1, 3, 10, 30, 100, 300 and 1000 µg L-1) were sent to Townsville Laboratory Services for dissolved copper quantification. Data was entered into excel.For full methodological and analytical details please refer to the full research publication, Thomas et al. (in press) its supplementary materials and the associated GitHub repository (GitHub - MarieCThomas/Copper.microbiome: Establishing guideline values for copper toxicity to marine microbiomes). Additionally, raw sequencing data can be found in the NCBI Sequence Read Archives under BioProject number PRJNA983933.

本数据集涵盖水质测量数据、营养盐数据与铜浓度实测数据，相关实验旨在探究铜对海水微生物群落的影响，以确立铜对海水微生物组的毒性指导值。本实验所用海水于2020年12月采自澳大利亚海洋科学研究所（Australian Institute of Marine Science, AIMS）码头（南纬19°16′38.4″，东经147°03′32.1″），随后开展了为期48小时的暴露实验。分别在实验起始0小时与结束48小时两个时间点，依照AIMS标准操作规程采集水质测量样本（含溶解氧、pH值与盐度）以及营养盐分析水样（用于检测溶解有机碳、溶解无机碳及氮）。将名义浓度梯度为0、0.1、0.3、1、3、10、30、100、300和1000 μg·L⁻¹的代表性实验处理组水样，送至汤斯维尔实验室服务中心开展溶解铜定量分析。所有数据已录入Excel电子表格。完整的实验方法与分析细节，请参阅研究论文Thomas等人（已付印）及其补充材料，以及配套的GitHub仓库（GitHub - MarieCThomas/Copper.microbiome: Establishing guideline values for copper toxicity to marine microbiomes）。此外，原始测序数据可在美国国家生物技术信息中心（NCBI）序列读取档案（Sequence Read Archives, SRA）中获取，对应生物项目编号为PRJNA983933。