Virus titres (pfu/ml) from the tissue culture supernatant of influenza virus-infected MDCK, CEF and A549 cells.
收藏Figshare2015-12-02 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_Virus_titres_pfu_ml_from_the_tissue_culture_supernatant_of_influenza_virus_infected_MDCK_CEF_and_A549_cells_/335442
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Each cell line was infected with each of the H1N1/WSN, H5N2/F118, H5N3 and H9N2 viruses using an MOI = 0.1 and 0.01 and incubated in DMEM containing 1 µg/ml TPCK trypsin and 0.21% BSA at 37°C. At 48 hpi the virus titres in the tissue culture supernatant were determined by agarose overlay plaque assay on MDCK cells. ND denotes no infectious virus particle detected. Representative data from one of two separate experiments is shown, and the average values are from duplicate measurements (SE
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2015-12-02



