Mitochondrial carrier SLC25A19 is required for respiratory chain function. Mitochondrial carrier SLC25A19 is required for respiratory chain function

In this study, we investigate how mitochondrial thiamine pyrophosphate (TPP) transporter SLC25A19 regulates mitochondrial function and cell metabolism. Overall design: We performed CRISPR-cas9 to knock out SLC25A19 in K562 cells using two different sgRNAs. Two knockout cell lines, SLC25A19-sg1 and SLC25A19-sg2, were selected and further used for phenotype analysis. We also used K562 cells with Non-targeting control sgRNA as a reference. RNA was collected after 48 h of culturing, and each cell line was performed in triplicate.