Fly Data - Aboufares et al BMC Biology 2023.zip

Fly data repository for Aboufares et al. BMC Biology 2023. This dataset contains raw and processed Drosophila sleep and wake activity of Linker flies and relative wild type flies. Fly stocks and crosses were maintained and tested on standard Drosophila medium at 25°C, 70% relative humidity with a 12h:12h LD cycle. For the experimental line UAS-linker flies were crossed to the pan-neuronal driver line elav-GAL4 (BL8760; Bloomington Drosophila Stock Center, IN, USA) and controls were generated by crossing Gal4 promoter and UAS responder lines to Canton-S w- (Dr Scott Waddell, University of Oxford, UK). Individual male progeny flies were collected between two to four days post eclosion and loaded in the Drosophila Activity Monitor system (DAM2, Trikinetics Inc., Waltham, MA, USA) placed inside a light- and temperature-controlled incubator. Locomotor data was collected in 1 min bins and sleep was defined as five or more minutes of inactivity. Sleep parameters were quantified in MATLAB using the SCAMP script. Baseline activity and sleep measurements were averaged over the four days of LD, and further split into the day (i.e. when lights were on) and night (i.e. lights off) components. Sleep deprivation was performed by placing the DAM monitors for both control and experimental flies on a shaker controlled by a timer. Flies were sleep deprived for the whole 12 h night phase of day five by activating the shaker for 5s in every 1 min and followed by a day of recovery. Flies that had died before the end of the experiment were removed from the analysis.