Maternal RNA transcription in Dlk1-Dio3 domain is critical for proper development of the mouse placental vasculature

Maternal RNA transcription plays important roles in maintaining vasculature in mouse placental development associated with regulating gene expression, imprinting status and DNA methylation in the Dlk1-Dio3 imprinted domain. We used Easi-CRISPR technology to insert three polyadenylation cassettes into the promoter of Gtl2 in the Dlk1-Dio3 imprinted domain to generate Gtl2 polyA knock-in mouse model. At embryonic day 12.5 (E12.5), the placentas of paternally Gtl2 polyA knock-in (PKI), maternally Gtl2 polyA knock-in (MKI), homozygous (HOMO) and wild-type (WT) mice were selected to do the RNA-sequencing.