Double Negative T Cells Downregulate Intrahepatic Inflammation and Ameliorate Hepatic Ischemia and Reperfusion Injury via CD39-adenosinergic axis [ChIP-seq]

Converted double negative T cells (cDNT) highly expressed ATP hydrolytic enzyme CD39 and hydrolyzed extracellular ATP (eATP) into adenosine. We found that adenosine upregulated the transcription factor FOXO1 expression in cDNT. CUT&Tag assay was performed to investigate the FOXO1 binding promoter sites in cDNT. Overall design: Converted double negative T cells (cDNT) were stimulated with 400µM adenosine with anti-CD3/CD28 antibody treatment in vitro for 6 hours. FOXO1 antibody, CUT & Tag Assay Kit were used to extract the binding site of FOXO1. Then the libraries were sequenced by Illumina Nova Seq 1.5 in Berry Genomics.