Effects of S100A8 inhalation on expression of genes in the lung microenvironment

Because S100A8 inhalation delayed lung tumor growth (LLC) in mice but did not directly affect tumor cell viability, we sought to determine potential changes in the lung microenviornment that contributed to its anti-tumor effects. Effects of S100A8 treatment on the lung microenvironment were assessed using a panel of 98 genes that affect immune regulation, redox, cancer growth, metastasis, hypoxia and angiogenesis. We averaged expression of each gene in lungs from 4-5 independent mice per treatment group. We compared gene expression in mice treated with S100A8+LLC, HBSS+LLC, S100A8+vehicle to HBSS+vehicle (control). In total, there were 4 samples.