Gene expression analysis of GD9.25 vehicle- or cyclopamine-exposed mouse frontonasal prominence and ventral prosencephalon tissues. Mus musculus

We sought to identify Hedgehog-regulated genes in the frontonasal process (FNP) and ventral prosencephalon at GD9.25 in mice, during the initial pathogenesis of cleft lip with or without cleft palate. Overall design: Frontonasal process and underlying ventral prosencephalon tissues were microdissected from GD9.25 (20-24 somite pairs) mouse embryos exposed to vehicle or cyclopamine from GD8.25-9.25. Tissues were pooled by litter to minimize intralitter variability. N=6 vehicle and N=6 cyclopamine pooled litters were included in the analysis.