Next Generation Sequencing Facilitates Quantitative Analysis of shPLKO- and shPSPH-Cancer Cell Transcriptomes. Next Generation Sequencing Facilitates Quantitative Analysis of shPLKO- and shPSPH-Cancer Cell Transcriptomes

We report the Phosphoserine Phosphatase (PSPH) would impair influence the Chemokines expression in TNF-A condition, Gene enrichment analysis of the transcriptome profiles of shPSPH-transfected hepatoma cells or shPLKO-transfected control cells could discover which changes in signaling pathways affect chemokine expression.PLC/PRF/5 and SNU449 were stimulated with human recombinant TNFα for 24 hours. RNA of the cells was extracted by TRIzol method. Overall design: Cancer cell line (PLC/PRF/5, SNU449) mRNA profiles of shPLKO (Contral) and shPSPH cell