Contributions of mirror-image hair cell orientation to mouse otolith organ and zebrafish neuromast function: Part 2/2, Hair cell and afferent physiology from mouse utricle

Otolith organs in the inner ear and neuromasts in the fish lateral-line harbor two populations of hair cells oriented to detect stimuli in opposing directions. The underlying mechanism is highly conserved: the transcription factor EMX2 is regionally expressed in just one hair cell population and acts through the receptor GPR156 to reverse cell orientation relative to the other population. In mouse and zebrafish, loss of Emx2 results in sensory organs that harbor only one hair cell orientation and are not innervated properly. In zebrafish, Emx2 also confers hair cells with reduced mechanosensory properties. Here, we leverage mouse and zebrafish models lacking GPR156 to determine how detecting stimuli of opposing directions serves vestibular function, and whether GPR156 has other roles besides orienting hair cells. We find that otolith organs in Gpr156 mouse mutants have normal zonal organization and normal type I-II hair cell distribution and mechano-electrical transduction properties. I..., We compared mechano-electrical transduction and whole-cell voltage-activated K+ currents in verstibular type I and type II hair cells from homozygous knockout (Gpr156–/–) animals and heterozygote (Gpr156+/–) littermates. We performed current clamp experiments to investigate firing properties of calyceal afferent nerves. , , # Contributions of mirror-image hair cell orientation to mouse otolith organ and zebrafish neuromast function: Part 2/2, Hair cell and afferent physiology from mouse utricle

[https://doi.org/10.5061/dryad.905qfttvj](https://doi.org/10.5061/dryad.905qfttvj)

##### Description of the data and file structure

This dataset is from the mouse utricle, comparing control tissue with tissue lacking the normal line of reversal of hair bundle polarity (LPR).  Data included are:  whole-cell patch clamp data on hair cell transduction and voltage-gated current properties (Figure 2 and its associated supplementary file 2, F2-FS2; Figure 3 and supplementary file 3, F3-FS3;  Figure 4 and supplementary file 4, F4-FS4) and vestibular afferent properties (Figure 8 and supplementary file 5, F8-FS5).   

Datasets are in excel format. Datasets have headers, and additional information is provided in this document.

##### DATA & FILE OVERVIEW

Each file corresponds to a parent figure and a Figure supplemen...