Transcriptomic analysis of liver mRNA from TSP-1 KO and WT mice fed with normal diet or choline deficient amino acid defined diet (CDAHFD, human NASH model)

Transcriptomic profiles of wild type and TSP1 knockout mice that were fed control (normal) diet or CDAHFD (choline deficient amino acid defiend high fat diet) chow for 12 weeks to model Non-alcoholic Steatohepatitis (NASH) disease in humans. Liver mRNA profiles of TSP-1 KO and WT mice that were fed control or CDAHFD (choline deficient amino acid defined high fat diet) chow for 12 weeks. (n=5 for each group). On day 0, C57/BL6 mice or TSP1 Knockout mice (JAX stock #006141) were provided with either Control chow (Research Diets A08051501) or CDAHFD chow (Choline deficient L-amino acid-defined high fat diet; Research Diets A06071309). After 12 weeks all groups were terminated with liver tissues were collected. One section of right medial lobe of liver was taken and snap-frozen in liquid nitrogen for RNA extraction. RNA was extracted with Trizol (Thermo Fisher Scientific # 15596026) after homogenization by Omni TH tissue homogenizer.