An R-based tool for identifying sex-linked markers from Restriction site-Associated DNA sequencing with applications to elasmobranch conservation

The data was collected to study genetic markers that are located on sex chromosomes and develop and R functions to efficiently identify such markers.The raw silico and SNP markers are provided. The Rmarkdown describes the workflow of the R function with the example White Shark data, the BLAST results of sex-linked markers against reference genomes, the conditions and results for the PCR-based sex identification, and the accuracy analysis of the function.Lineage: The SNP data were created following the DArTseq protocol (Diversity Arrays Technologies, Canberra).

本数据集的采集旨在研究位于性染色体上的遗传标记，并开发可高效识别此类标记的R语言函数。原始计算机模拟数据与单核苷酸多态性（Single Nucleotide Polymorphism, SNP）标记已随数据集一并提供。配套的R Markdown文档阐述了该R语言函数的工作流程，内容涵盖大白鲨示例数据集、性连锁标记与参考基因组比对的局部序列比对基本工具（Basic Local Alignment Search Tool, BLAST）结果、基于聚合酶链式反应（Polymerase Chain Reaction, PCR）的性别鉴定条件与结果，以及该函数的精度分析。数据谱系：本数据集的SNP标记遵循DArTseq技术流程（Diversity Arrays Technologies, 堪培拉）生成。