Transcriptomic profiling of the PhzIR quorum sensing, Gac- and PtrA- regulons of Pseudomonas chlororaphis PA23

The biocontrol agent Pseudomonas chlororaphis PA23 protects canola (Brassica napus) against infection by the necrotrophic fungus Sclerotinia sclerotiorum. Production of PA23 secondary metabolites is governed by a complex regulatory pathway that includes the GacA/GacS two component system, the PhzI/PhzR quorum-sensing system, and a novel LysR-type transcriptional regulator, called PtrA. Through RNA-sequencing, transcriptomic profiles of PA23-WT, two quorum sensing-deficient strains, PA23-AHL and PA23-phzR, and regulatory mutants PA23-gacA, PA23-gacS and PA23-ptrA were generated allowing elucidation of the PhzRI, Gac and PtrA regulons of P. chlororaphis PA23. a total of 18 samples: 6 strains: Pseudomonas chlororaphis PA23 Wildtype (control), gacA-, gacS-, ptrA-, phzR- and AHL- deficient strains with 3 biological replicates per strain