Expression data from Lund Human Mesencephalic cells (LUHMES) exposed to acute and persistent oxidative DNA damage

Oxidative DNA damage in neurons activates a DNA damage response (DDR) to promote repair. Under a chronic oxidative environment these processes may be altered and promote accumulation of unrepaired DNA damage and continued activation of a DDR, leading to apoptosis or senescence activation. Accumulation of oxidative DNA damage are features of brain ageing and neurodegeneration but the effects of persistent DNA damage in neurons are not well characterised. We developed a model of persistent oxidative DNA damage in human neurons in vitro (LUHMES) by exposing them to a sub-lethal concentration of hydrogen peroxide (H2O2) following a "single stress" and “double stress” protocols. We characterised the neuronal transcriptome under these circumstances using microarray analysis. Single-stressed post-mitotic LUHMES in culture were exposed to 50 μM H2O2. Double-stressed post-mitotic LUHMES were exposed to a second 50 μM H2O2 stress 6 hours after the first one. Control LUHMES were exposed to vehicle (distilled water). LUHMES under the three different conditions were kept in culture for a further 96 hours and harvested at this time-point for total RNA isolation and microarray analysis.