Data for: SNPs detected in pool-seq data from resistant and susceptible Cimex lectularius populations

In the last few years, the bed bug Cimex lectularius has been an increasing problem world-wide, mainly due to the development of insecticide resistance to pyrethroids. The characterization of resistance alleles is a prerequisite to improve surveillance and resistance management. To identify genomic variants associated with pyrethroid resistance in Cimex lectularius, we compared the genetic composition of two recent and resistant populations with that of two ancientsusceptible strains using a genome-wide pool-seq design. We identified a large 6 Mb \"superlocus\" showing particularly high genetic differentiation and association with the resistance phenotype. This superlocus contained several clustered resistance genes, andwas also characterized by a high density of structural variants (inversions, duplications). The possibility that this superlocus constitute a resistance \"supergene\" that evolved after the clustering of alleles adapted to insecticide and after reduction in recombination is ..., The four strains used in this studywere provided by CimexStore Ltd (Chepstow, United Kingdom). Two of these strains were susceptible to pyrethroids (S), as they were collected before their massive use and have been maintained under laboratory condition without insecticide exposure for more than 40 years : German Lab (GL, collected in Monheim, Germany) and London Lab (LL, collected in London, Great Britain). The other two resistant (R) populations were London Field (LF, collected in 2008 in London) moderately resistant to pyrethroids, and Sweden Field (SF, collected in 2015 in Malm., Sweden), with a moderate-to-high resistance level.
For each strain, genomic DNA was extracted from 30 individual females (except for London Lab which had only 28) using NucleoSpin 96 Tissue Kit (Macherey Nagel, Hoerdt, France) and eluated in 100 μL of BE buffer. DNA concentration of these samples was measured using Quant-iT PicoGreen Kit (ThermoFisher, Waltham MASS, USA) according to manufacturer’s instructi...,