Data for: SNPs detected in pool-seq data from resistant and susceptible Cimex lectularius populations

In the last few years, the bed bug Cimex lectularius has been an increasing problem world-wide, mainly due to the development of insecticide resistance to pyrethroids. The characterization of resistance alleles is a prerequisite to improve surveillance and resistance management. To identify genomic variants associated with pyrethroid resistance in Cimex lectularius, we compared the genetic composition of two recent and resistant populations with that of two ancientsusceptible strains using a genome-wide pool-seq design. We identified a large 6 Mb \"superlocus\" showing particularly high genetic differentiation and association with the resistance phenotype. This superlocus contained several clustered resistance genes, andwas also characterized by a high density of structural variants (inversions, duplications). The possibility that this superlocus constitute a resistance \"supergene\" that evolved after the clustering of alleles adapted to insecticide and after reduction in recombination is ..., The four strains used in this studywere provided by CimexStore Ltd (Chepstow, United Kingdom). Two of these strains were susceptible to pyrethroids (S), as they were collected before their massive use and have been maintained under laboratory condition without insecticide exposure for more than 40 years : German Lab (GL, collected in Monheim, Germany) and London Lab (LL, collected in London, Great Britain). The other two resistant (R) populations were London Field (LF, collected in 2008 in London) moderately resistant to pyrethroids, and Sweden Field (SF, collected in 2015 in Malm., Sweden), with a moderate-to-high resistance level. For each strain, genomic DNA was extracted from 30 individual females (except for London Lab which had only 28) using NucleoSpin 96 Tissue Kit (Macherey Nagel, Hoerdt, France) and eluated in 100 μL of BE buffer. DNA concentration of these samples was measured using Quant-iT PicoGreen Kit (ThermoFisher, Waltham MASS, USA) according to manufacturer’s instructi...,近数年来，温带臭虫（Cimex lectularius）已成为全球范围内日益严峻的公共卫生问题，其主要诱因是该物种对拟除虫菊酯类杀虫剂产生了抗药性。对抗性等位基因进行表征，是优化监测体系与抗性管理策略的必要前提。为鉴定温带臭虫（Cimex lectularius）中与拟除虫菊酯抗性相关的基因组变异，本研究采用全基因组混池测序（pool-seq）设计，对比了两株新近采集的抗性种群与两株古老敏感品系的遗传组成。研究团队鉴定出一个长达6 Mb的“超级位点（superlocus）”，该位点呈现极高的遗传分化水平，且与抗性表型显著相关。此超级位点包含多个成簇分布的抗性基因，同时还具有高密度的结构变异（倒位、重复）。该超级位点有可能是一类抗性“超级基因（supergene）”，其演化过程或与适配杀虫剂的等位基因成簇、重组率降低相关…… 本研究使用的4个品系均由英国切普斯托的CimexStore有限公司提供。其中两株为拟除虫菊酯敏感品系（S），采集于该类杀虫剂大规模使用之前，且已在无杀虫剂暴露的实验室条件下饲养超过40年：分别为德国实验室品系（German Lab, GL，采集自德国蒙海姆）与伦敦实验室品系（London Lab, LL，采集自英国伦敦）。另外两株为抗性种群（R）：伦敦野外种群（London Field, LF，2008年采集于伦敦）对拟除虫菊酯具有中度抗性，瑞典野外种群（Sweden Field, SF，2015年采集于瑞典马尔默）则表现出中至高抗性水平。 对于每个品系，均从30只雌性成虫中提取基因组DNA（伦敦实验室品系仅获取28只），使用NucleoSpin 96 Tissue Kit（Macherey Nagel, 法国奥尔杜）进行提取，最终用100 μL BE缓冲液洗脱。采用Quant-iT PicoGreen Kit（ThermoFisher, 美国马萨诸塞州沃尔瑟姆）测定样本的DNA浓度，操作严格遵循制造商提供的操作说明……