The stabilities of non-canonical structures designed to form in template DNA a

aAll experiments were carried out in a buffer containing 30 mM KCl, 40 mM Tris-HCl (pH 8.0), 8 mM MgCl2, and 2 mM spermidine.bThe sequences of template DNAs are shown in Figure 1b and Table S1 in File S1. The sequences designated by lower case letters contain only the non-canonical structure region (see Table S2 in File S1).cThe melting temperature was determined at a strand concentration of 2 µM.dArrest was defined as more than 4% production of arrested product RNA.eThe −ΔGo37 value could not be determined because of very high stability.