HOS15 and HDA9 negatively regulate immunity through histone deacetylation on intracellular immune receptor NLR genes in Arabidopsis

We isolated AtHOS15 as a negative regulator of plant immunity from genetic screen. To investigate how HOS15 influences plant immunity, we performed whole genome transcriptome (RNA-seq) analysis of the hos15-4 mutant. A total of 3,512 differentially expressed genes, with 1,870 upregulated and 1,642 downregulated, were found in hos15-4 mutant compared to wile-type Col-0. Gene Ontology analysis showed that genes involved in defense responses including immune receptor NLR genes are highly enriched among upregulated genes. Overall design: Examination of whole genome transriptome (RNA-seq) in hos15-4 mutant compared to wide-type Col-0. Two-week-old plants growing under constant light at 22C were used for RNA extraction.