A novel strategy for regulating mRNA’s degradation via interfering the AUF1’s binding to mRNA [RIP-seq]

Purpose: To verify the relationship between AUF1 and tumor cells proliferation, we detected the function of AUF1 in Hela cells using RIP-seq analysis. Methods: Genomic alignment (version from UCSC genome browser) was using Tophat (version:2.0.13) to get uniquely mapping reads. p value <0.05 considered as significantly modulated, were retained for further analysis. This choice is motivated by the decision to maximize the sensitivity of this analysis, in order to perform a massive screening and identify candidate genes to be validated with a wider sample population with real-time PCR analysis Results: The quantity of gene expression was calculated by FPKM (Fragments Per Kilobase of transcript per Million fragments mapped).50% of the binding region of AUF1 was in the exon region, and others were mainly in the 3’-UTR regions (29.6%). AUF1’s targeted genes were related to metabolic and single-organism processes. AUF1’s targeted genes were mainly concentrated in the protein synthesis process of the endoplasmic reticulum and cancer pathway. Conclusions: AUF1 might be involved in the regulation of translation and participated in the regulation of tumorigenesis and development through its target genes. Fragment of RNA binding to AUF1 antibody in Hela cells