METHOD FOR SEPARATING AND PURIFYING RECOMBINED HUMAN LACTOFERRIN FROM RICE SEEDS
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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资源简介：
The present invention， (1) using a transformed rice seed with recombinant human lactoferrin as a raw material， using the extraction buffer to prepare a crude rLF extract; And (2) separating and purifying the rLF crude extract by cation exchange chromatography to obtain a purified rLF target object， a chromatographic method for isolating and purifying recombinant human lactoferrin from transformed rice seeds. to provide. The chromatography method further includes regenerating the resin. The present invention provides a chromatographic method for separating and purifying recombined human lactoferrin from transgenic rice seeds， comprising the following steps: (1) by using transgenic rice seeds with recombined human lactoferrin as a raw material， using an extraction buffer to prepare an rLF crude extract; and (2) separating and purifying the rLF crude extract by cation exchange chromatography， to obtain a purified rLF target object. The chromatographic method further comprises a feeding and regenerating step. The present invention provides a chromatographic method for separating and purifying recombined human lactoferrin from transgenic rice seeds， comprising the following steps: (1) by using transgenic rice seeds with recombined human lactoferrin as a raw material， using an extraction buffer to prepare an rLF crude extract; and (2) separating and purifying the rLF crude extract by cation exchange chromatography， to obtain a purified rLF target object. The chromatographic method further comprises a feeding and regenerating step.
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
