Trichoderma harzianum Transcriptome or Gene expression. Trichoderma harzianum

After the total RNA of the sample is extracted, the eukaryotic mRNA is enriched with magnetic beads with oligo (DT) (if it is a prokaryote, remove the rRNA with a kit and go to the next step). Fragment buffer was added to break the mRNA into short fragments. Using mRNA as template, the first cDNA strand was synthesized with six base random primers (random hexamers), and then buffer, dNTPs, RNase H and DNA polymerase I were added to synthesize the second cDNA strand. After purification by qiquick PCR kit and elution with EB buffer, terminal repair was performed, poly (a) was added and sequencing connector was connected, Then, agarose gel electrophoresis was used to select the size of the fragments. Finally, PCR was amplified. The sequencing library was sequenced by Illumina HiSeq.