MVA deletion mutants.

MVA deletion mutants used in this study. Different clusters of ORFs were deleted from the mutants, encoding proteins with different functions (mentioned with references). In a few cases, there were no known functions associated with ORFs. The mutants express the 85A or TIP antigens that were inserted at the TK locus. All mutants were made using MVA-BAC recombineering, therefore, all contain BAC DNA and GFP (green fluorescent protein) marker.
aTK: Thymidine kinase locus, used as an insertion site for the recombinant antigens.
bVACV COP: Vaccinia virus Copenhagen strain.
cWR: Vaccinia virus Western Reserve strain.
dThe ORF B15R is the MVA184R gene. It is named B15R in VACV WR while it is B16R in VACV COP. This made inconsistency in reporting this ORF as well as the downstream B fragment ORFs in the literature. Here, we report MVA184 as the B15R, consistent with our previous work [22], and in accordance with another report [11]. However, it was reported as B16R in another study [13]. This ORF encodes IL-1β binding protein [11].
MVA deletion mutants.