Optogenetic control of the integrated stress response reveals proportional encoding and the stress memory landscape. Optogenetic control of the integrated stress response reveals proportional encoding and the stress memory landscape

To understand ISR signaling we engineered photo-switchable control over the ISR stress sensor kinase PKR (opto-PKR), which allows virtual control of the ISR. Using controlled light inputs to activate opto-PKR we traced information flow in the ISR both globally, in the transcriptome, and for key ISR effectors Overall design: To investigate transcriptomic dynamics of opto-PKR-induced ISR over time, H4-cells bearing opto-PKR were illuminated using the optoPlate device to collect 7 samples across 720 mins.