Reproducible single cell annotation of programs underlying T cell subsets, activation states, and functions

CITE-sequencing and TCR-sequencing of sorted activated, unactivated, and mock T cells derived from blood. T cells from five healthy human donors were stimulated with a peptide pool (CEFX, JPT) and anti-CD28/CD49d co-stimulation. Cells were then sorted based on activation (OX40 and PD-L1 for CD4s, CD137 for CD8s) and labeled with corresponding hashing antibodies prior to sequencing. In total, this dataset encompasses 42,370 cells (12,743 AIM-positive, 15,369 AIM-negative, 14,258 Mock). CITE-sequencing and TCR-sequencing of sorted activated, unactivated, and mock T cells derived from blood. T cells from five healthy human donors were stimulated with a peptide pool (CEFX, JPT) and anti-CD28/CD49d co-stimulation. Cells were then sorted based on activation (OX40 and PD-L1 for CD4s, CD137 for CD8s) and labeled with corresponding hashing antibodies prior to sequencing. In total, this dataset encompasses 42,370 cells (12,743 AIM-positive, 15,369 AIM-negative, 14,258 Mock).