Dengue specific immunoglobulin repertoires comparison between the repeated and epitope-decreased immunizations

This study aims to under the characteristics and diversitification of Dengue speicific immunoglobulin repertoires after different immunization strategies in mouse. We propose two different immunization strategies in dengue vaccine development, one is repeated strategy, which DENV1 virus and administrated three times. The other one is epitope-decreased sequential strategy, which sequetially administrated DENV1 virus, DENV1 E protein and DENV1 E protein domain III subunit. Overall design: C57BL/6J (B6) mice were injected with either repeated or sequential strategy. The Dengue specific B cells were fished out from immunized mice splenocytes by Dengue Env protein probes. After cDNA and Ig specific amplification, the RNA-seq libraries were preparated and subjected to MiSeq V3 2 × 301 bp sequencing.