The circadian clock protein Cryptochrome 1 is a direct target and feedback regulator of the Hippo pathway

To examine the impact of Cry1 downregulation on gene expression, empty vector (Sh Ctrl) and Sh-RNA targeting Cry1 (Sh Cry1) were used to produce lentiviral vector. Cell were then infected with control and sh-RNA lentivirus and then selected with puromycin. Upon validation of the knockdown with real time PCR. RNA were prepared from cells stably expressing empty vector or sh-RNA against Cry1 and used gene expression profiling by RNA seq. RNA of two biological replicate were prepared from each genotype. To examine the impact of Cry1 downregulation on gene expression empty vector (Sh Ctrl) and Sh-RNA targeting Cry1 (Sh Cry1) were used to produce lentiviral vector.