Genome-wide studies identify the role of PML/RARα phase separation in PML/RARα-driven transcriptional dysregulation.

Acute promyelocytic leukemia (APL) is characterized by a specific t(15;17) chromosome translocation that generates the promyelocytic leukemia/retinoic acid receptor-α (PML/RARα) fusion gene. However, the mechanism underlying PML/RARα mediated transcriptional dysregulation remain unclear. Here, we performed the transcription profiling in NB4, an APL patient-derived cell line. Expression profiling of NB4 cells with endogenous PML/RARα knock-down and replaced with empty vector or full-length PML/RARα or phase separation-imcompetent mutants (F158E or ΔIDR) using RNA-seq.