ChIP after EBF1 degradation

We used the dTAG protein degradation system to rapidly deplete transcription factor EBF1 in the B cell culture. After treatment with dTAG-13 for 6 hours, we performed ChIP-seq, ATAC-seq, and RNA-seq. After the treatment, EBF1 is completely absent from the chromatin. This leads to the closure of EBF1 binding sites and changes in the target gene expression. This approach allowed us to identify direct targets of EBF1 and show the importance of EBF1 for open chromatin maintenance. ChIP-seq with a-EBF1 (1C) antibody in EBF1-dTAG_C proB cells before and after 6 hours of treatment with dTAG-13. ChIP-seq with a-Brg1 (ab110641) antibody in EBF1-dTAG_N proB cells before and after 6 hours of treatment with dTAG-13. There are ChIP sample and input for each condition.