Regulatory T cell-specific transcriptional regulation

To determine the regulatory T cell-specific transcriptional regulation, we compared gene expression profiles of regulatory T, na?ve T and activated conventional T cells. As activated T cells, we prepared two sets of them: conventional T cells stimulated with anti CD3 and CD28 antibodies, and those stimulated by PMA and ionomycin. Comparison of these cell types should elucidate activation-related and regulatory T cell lineage-specific transcriptional programs.